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Microbiology Project Topics

Incidence of Candidacies Among Singles Girls and Married Women Attending St John Healthcare Center, Rumuolumeni

Incidence of Candidacies Among Singles Girls and Married Women Attending St John Healthcare Center, Rumuolumeni

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Incidence of Candidacies Among Singles Girls and Married Women Attending St John Healthcare Center, Rumuolumeni

Chapter One

Aimย Andย Objectives

Toย determineย theย prevalenceย ofย fugalย infectionย amongย Singles girls And Married women attendingย St John health Center, Rumuolumeni state

  1. To determine the prevalence of fungal infection among single and married women of different age group patient
  2. To determine the yeast type
  3. To determine the antimicrobial sensitivity pattern of isolates

CHAPTERย TWO

LITERATURE REVIEWย 

Candidiasis

According to Haymer et al. (2019), cultured fungi were discovered in 71% of mouth smear samples taken from patients who were presenting at a dermatology clinic for the cadida krusei. Candida albicans is widespread and can be found in many healthy people. Mothers with candidiasis of the vaginal trait may infect their newborns with Candida tropical Candida pseudoropicals and other species of Candida (woodrute et al 2019) Nembutal candidosis (Moniliasis) A perleche brought on by a lack of nutrients, B angular cheeks (2015).

Up to 40% of newborn infants’ mouths have been shown to contain Candida albicans, which often manifests itself between the sixth and seventh postpartum (Tesehsian et al 2015).

However, the presence of Candida albicans does not imply that the infection must be facilitated by a number of circumstances, including age (infants elderly persons health status (diabetes pregnant and have dirty moreover local factors are significant, such as ede tulousuess.

In recent years, the extensive therapeutic use of antibiotics has led to an increase in Candida infections of the mouth, respiratory, digestive, and skin, especially in the anogenetal area. As a result, candidiasis is currently one of the most frequently occurring diseases in my body due to fitting denatures, and generally lowered body resistance due to conditions like molabsorption system malignancy uremia, and various chronic infections (Lighterman et al 2016).

clinical traits Oral candidiasis can be localized as superficial morihial matous candidiasis or diffuse as angular chenlosis (Ketchum 2016).

Patients who have a habit of licking their hips after having peri-oral chronic eczema from which mantilla organisms can be cultured are more likely to develop diffuse monclial cheibosls, which is characterized by shiny red erosion with desquamation of the epithelium and surrounding hyperkeratosis. In the granulating type, there is diffuse swelling of the lowest hip or, less frequently, both hips.

Angularย cleilosisย (Marginalย cellistsย perlecheย characterizeย byย symmetricalย lesions at the angles of the mouth is most frequently caused or hundred inย leadingย byย candidย celbicanusย (Finnenidย etย alย 2019)ย variousย factorย such decreasedย verticalย lightย dueย toย theย eddeutalousย stateย illย fittingย denturesย orย flaccid sagging cheeks and depressed habial angles constemitly moistened byย saliva play an important role ย ย in the origin of these trouble some tissuredย lesions perleche may also be observed in patients with achlyha hypochrorunicย anemiaย or hypouitamimosisย B.

In superficial monclial stomatitis the chricl piture ranges form mild crythemcย with fineย whitishย deposits toย diffuseย inflamed whiteย mouth.

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Inย infantsย theย firstย changesย appearย onย theย anteriorย dorsalย thirdย edgesย andย ventralย surfaceย onย theย andย laterย inย theย oralย vestibuleย theย lesionsย resemblingย snow white curdled mulk (Taylor 2018) can present as strips plagues andย diffuseย pseudomenbranesย andย usuallyย canย beย strippedย muchย easierย thanย diptheptia meubrance generally the easier the mechanical removes the moreย superficial and less harmful the epithelial immersion by pelezar J.M the whiteย spot are composed of a deuse matting of candid albicans together with cellย detritus residual food particlesย and bacteria the surface of the lesions has aย valvesty appearance where as the adjacent mucous membrane appears durk redย moderatelyย swolleaย alceratureย orย necroticย lesionsย indicateย deeperย tissueย in version an unfavorable prognostic sign enthrocolitis mycosis pneumonia orย sppticemeย rarelyย mayย occur.

In deutre stomatitistis the patient complains of swelling senstaruly and pain theย oral mucus membrane atย point of denture contacts, numerous colonies ofย candida aibicanis are found on the inflamed mucosa not uncommonly thereย exist sinuitaneous predisposition disorders such as diabetses mellitus anemiaย malnutritionย orย digesterย disturbancesย candidaย interactionย mayย secondarilyย complicateย oral peaphigus and otherย process (Leuwenย hockย 2018)

As previously stated the wider spread use of antibiotic therapy poses a seriousย problem in combating lateiod Cndida saprophytism thus in addition to the riskย of seusitizatio is the man reason for discouraging the use of tropical antibioticsย for local therapy of oralย \lesions Maywa Rohn (2019) candidiasis also mayย been in associationย withย acrodermater enteropathica.

In deep granuhomatous candidiasis there are deep invasion by the monclialย organismย formationย ofย theย tuberculoidย granulomasย thisย appearsย toย theย anย autosmer recessure trait it may occur in association with hypoarathy and withย addisons disease.

The infection usually begging in a relatively harmless form in the oral cavity ofย candidiasis (Momliasis) a candida tropiculis associated with vegeners granuloย matosis B thick blanket of moruliail organisms on under surface of tongue byย Buchoiz (2010) candida, tropicalis mylose wegenersde granu homatose. Newย born infants and children during the first years of life , it then spread to the faceย andย extendsย overย theย entireย head,ย creatingย inflratedย crustedย plaqueย andย developingย seversย granuloย matousย reassureย inย theย tissuesย thatย reactsย theย intensityย ofย granulonutonย blasย toย myย cosisย theย lipsย appearย swollenย withย papilhomatos proliferation and radiating tissues well developed perleche isย found on the moist argues of the mouth.

 

CHAPTERย THREE

METHODOLOGYย ANDย MARTERIALS

INTRODUCTION

Theย specimenย bottleย forย urineย collectionย theย petriย dishesย forย mediaย preparation and all the test tubes were all washed and sterilized in the hot airย ovenย atย 160cย forย 1ย hourย andย keptย sterileย untilย usedย inย theirย respectureย canisters.

POPULATIONย SAMPLED

Patients showing clinical symptoms of fungal infection and those onย antibiotic therapy ere excluded from the exercise by this, I was intended thatย candidiasis case of fungal infection and false negative results would be avoidedย respectively midย streamย urineย sampleย andย husย samplesย wereย collectedย fromย totalย ofย eighty (80) hospital patients (both single and married of university Nigeria teachingย hospital in hematology laboratory with age range of 10to 30 for single womenย andย 20 to 40 yearsย ofย ageย married women duringย theย period August 2005.

PREPARATIONย OFย CULTUREย MEDIA

Allย theย cultureย mediaย wereย commerciallyย obtainedย andย preparedย according to the manufacturers instruction., the mediu was sterilized conicalย flasks plugget with cestton wool and autoclaved for 15 min at 121c at is 16sย pressureย afterย autoclaving theย mediaย wereย keptย sterileย untilย used

ย COLLECTIONย OFย SAMPLES

Sterile universal container with measure quality of boric acid (0.29) wereย given to the selected patients. They were instructed on how to collect earlyย morning mid streamย unne to avoid contamination samples once collected wereย taken to the laboratory for analysis while Hvs sample were also collected fromย theirย vaginalย for analysis at theย sameย time.

LABORATORYย PROCESSINGย MACROSCOPY

Urine samples collected wereย microscope examined and their colourย recorded respture normal freshly passed urine is clear and pa yellow to yellowย depending on concentration. Any shift from thus normal is indication of someย diseaseย condition.

CHAPTERย FOUR

RESULTS

out of a total of eighty 180 samples screened 30 gouge significant yeast diseaseย these represent an incidence rate of 40% percent among single and marriedย women in St John health Center, Rumuolumeni state the most predominant organism was C albicans C.ย tropical Cย pseudotropicals Cย krueseiย Cย parasilosis

CHAPTER FIVE

DISCUSSION AND CONCLUSION

ย Discussion

Treatment of candida infections. a variety of agents are available forย treatingย the different typesย ofย candida infections. system orย internalย organย involvementย can be treatedย successfully withย amphotericin bย unfortunatelyย patientย withย immuneย deficienciesย wellย routinelyย becomeย reinfectedย nightstandย is usually effectiveย for the treatment of mucocutanous candidiasis such asย thrush or vagintis other antibiotics used for the treatment ofย mucoutaneusย candidiasis include mc camisole and chotrimoie oral and coetaneous infectionย also respondย weltย aย 1ย %ย solutionย ofย gentianย violet newer therapeutic measure are in all likelihood responsible for the increase inย candidaย infection seen over theย pastย couple ofย suppress theย normalย floraย undoableย isย responsibleย forย increasedย numberย ofย infectionย particularlyย gastrointestinal candidiasis. the used of polyethylene cutlets and implantedย prostheticย devices isย alsoย associated with increasedย candidaย infection.

NATURALย TREATMENTย FORย CANDIDIASIS

If possible after consultation with your doctor eliminate antibiotics birthย control pillsย corticosteroides andย uicerย drugs.

Being a yeast and sugar free diet and follow it stretchy for at least thirty days ifย yourย symptomsย diminish.ย Youย knowย thatย youย haveย beenย sufferingย fromย candidiasisย depending on the severity of your symptoms you will have toย follow the yeast free diet for three to twelve mouths ehimmate the followingย foods bread baked goods cheese mushrooms vinegar, soy source fermentedย food alcohol, also eliminates sugar including all sweets cookies, candy iceย cream, soda diet soda dried fruits,ย chocolate and sweetener including fructose,ย malt, barley and fruit juice lactobacillus acidophilus: inย natural form in yogurtย make sure the yogurt contains live cultores as indicated on the label and inย capsule form available in health food stores, tale one capsule three time dairyย garlic. In its natural form or in capsule form available from health food storesย capryficย acid followย the directions on theย container asย toย dosage In addition: there is a prescription drug called nystatin that can be of help toย confirmedย casesย ofย candidiasisย ifย youย tryย theย yeastย freeย dietย andย yourย symptoms are alleviatedย butย do notย completelyย clear.ย Themย you might haveย has candidiasis for so long that you will require additional treatment in the form ofย prescription drugย you shouldย discuss Nysktin with yourย doctor.

The prevalence of vaginal candidiasis in pregnant women was so high especially in the age ranging from 31 to 35 years and Candida albicans was the most prevalence vaginal candidiasis across the age group. Therefore, determination of the prevalence of candidiasis and occurrence of vaginal candida species in pregnant women will of importance in diagnosis of the infection and giving better antenatal service in the country.

RECOMMENDATION

The screening of candidiasis infection among single and married womenย of differentย age group thisย workย has givenย anย indicationย thatย mostย of theย women many suffer form candida infection in future. It established the fact thatย in every 80 patients 40 percent of them may be four with significant to theย disease in incidence is higher in single women than in married women it isย therefore recommended that the planners our health care delivery programย shouldย includeย screeningย programeย forย theย injectorย among youngย girls.

The federal government should also help to fund the teaching and federalย hospitals so that the screening should be carried out in affordable price also theย federal government should help to provide some thing like lecture on televisionย screen about the causes, consequencesย and natural treatment on candidiasisย infection.

In view of the high prevalence of vaginal infections, pregnant women attending antenatal clinic should have prompt and adequate diagnosis with appropriate treatment to prevent adverse effect of the infection on mother and foetus. Also, personal hygience and environmental sanitation which include not sharing of towels, wearing clean underwears, cleaning of bathroom and toilets, etc should be done regularly. In addition, a comprehensive programme on reproductive healthcare education with the aim of reducing vaginal infection prevalence should be put in place.

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